Standardization (External and Internal) of HPLC Assay for Plasma Homocysteine

Standardization (external and internal) of HPLC assay for plasma homocysteine.

Measurement of plasma homocysteine may be of value in several clinical conditions including homocystinuria, ath-erosclerosis, thrombophilia, and folate/vitamin B 12 deficiency. The increasing interest in measuring total homo-cysteine in plasma has led to the development of several different methods [1]. A widely used technique for measuring total plasma homocysteine is reversed-phase HPLC with ...

Method comparison for total plasma homocysteine between the Abbott IMx analyzer and an HPLC assay with internal standardization.

for clinical laboratory use. The method is simple and precise, enabling comparatively easy processing of large numbers of specimens. The performance characteristics of the method presented here are comparable with those utilizing fluorescence detection. The major advantage of this method is the avoidance of derivatization. The use of penicillamine as an internal standard for HCySH analysis had ...

Assay of serum fructosamine: internal vs external standardization.

2. Galen RS. The enzyme diagnosis of myocardial infarction. Hum Pathol1975;6:14155. 3. Lott JA. Creatine kinase, lactate dehydrogenase and their isoenzymes in clinical medicine. Corning, NY: Corning Medical, Corning Glass Works, 1984. 4. Yusuf S, Collins R, Lin L, Sterry H, Pearson M, Sleight P. Significance of elevated MB isoenzyme with normal creatine kinase in acute myocardial infarction. Am...

Rapid and accurate HPLC assay for plasma total homocysteine and cysteine in a clinical laboratory setting.

Measurement of Plasma Total Homocysteine by HPLC

23 mg/L for the evaluation tube, with an average difference between tube types of 35 6 4.3 mg/L. The test results for blood lead and EP on samples collected in the evaluation tubes were clearly equivalent to those collected in the independently certified, lead-free control tubes. The availability of pediatric pretested lead-free collection tubes will contribute to accurate and reliable diagnosi...